Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Biol. Res. (2) BJMBR (2) MV&Z (1) BABT (1) Genet. Mol. Biol. (1) Autor AGUIRRE,PABLA (1) ARACENA,PAULA (1) Alves,M.A.V.R. (1) Bian,Qi (1) Camargo,Matheus (1) Carvalho,H. (1) Costa,F.F. (1) Daudt,Liane (1) Doneda,Divair (1) Higino de Campos, Karla Conceição (1) Jin,Gang (1) Jing,Wei (1) Keyhanvar,Neda (1) Kohayagawa, Aguemi (1) Koppe,Tiago (1) Li,Gang (1) Liu,An-An (1) Liu,Rui (1) MUÑOZ,PABLO (1) Meneghini,R. (1) Mais... Palavra-chave Iron metabolism (7) Anemia (2) Biomarkers (1) Bone marrow Metabolismo del hierro (1) Chaperonins (1) Chronic renal insufficiency (1) Ferritin (1) Gaucher disease (1) Gene expression (1) Glutathione metabolism (1) GroEL (1) GroES (1) HAMP (1) HFE (1) Hematologia (1) Hematology (1) Hematología (1) Hepatocyte (1) Hepcidin-25 (1) Iron accumulation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (2) Info:eu-repo/semantics/other (1) Ano 2016 (1) 2015 (2) 2008 (1) 2006 (1) 2005 (1) 2002 (1) Mais... País Brazil (5) Chile (2) Idioma Inglês (6) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última Cloning and Functional Assessment of the Recombinant Human Hepcidin-25 in the Baculovirus Expression System BABT Yazdani,Yaghoub; Keyhanvar,Neda; Tabaraei,Alijan. Hepcidin is the primary regulatory hormone responsible for lowering the iron content in the blood circulation. Due to its biodegradability and low cytotoxicity, hepcidin is considered as an alternative for iron chelators. The baculovirus expression system may be suitable for human hepcidin production because the expressed proteins generally exhibit proper folding, post-translational modifications, and oligomerization. Using data from two vector maps, pFastBac1 and pFastBac HTB, a unique vector was designed encoding human hepcidin-25 as fusion recombinant peptide. Expression analysis showed that it was expressed as a peptide with a molecular weight near to 5 kDa. After purification and TEV treatment, findings revealed that recombinant human hepcidin-25 was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepcidin-25; Iron metabolism; SF-9 expression system. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100090 Hemochromatosis (HFE) gene mutations in Brazilian chronic hemodialysis patients BJMBR Perícole,F.V.; Alves,M.A.V.R.; Saad,S.T.O.; Costa,F.F.. Patients with chronic renal insufficiency (CRI) have reduced hemoglobin levels, mostly as a result of decreased kidney production of erythropoietin, but the relation between renal insufficiency and the magnitude of hemoglobin reduction has not been well defined. Hereditary hemochromatosis is an inherited disorder of iron metabolism. The importance of the association of hemochromatosis with treatment for anemia among patients with CRI has not been well described. We analyzed the frequency of the C282Y and H63D mutations in the HFE gene in 201 Brazilian individuals with CRI undergoing hemodialysis. The analysis of the effects of HFE mutations on iron metabolism and anemia with biochemical parameters was possible in 118 patients of this study (hemoglobin,... Tipo: Info:eu-repo/semantics/other Palavras-chave: HFE; Chronic renal insufficiency; Anemia; Iron metabolism. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900005 Increased expression and purification of soluble iron-regulatory protein 1 from Escherichia coli co-expressing chaperonins GroES and GroEL BJMBR Carvalho,H.; Meneghini,R.. Iron is an essential metal for all living organisms. However, iron homeostasis needs to be tightly controlled since iron can mediate the production of reactive oxygen species, which can damage cell components and compromise the integrity and/or cause DNA mutations, ultimately leading to cancer. In eukaryotes, iron-regulatory protein 1 (IRP1) plays a central role in the control of intracellular iron homeostasis. This occurs by interaction of IRP1 with iron-responsive element regions at 5' of ferritin mRNA and 3' of transferrin mRNA which, respectively, represses translation and increases mRNA stability. We have expressed IRP1 using the plasmid pT7-His-hIRP1, which codifies for human IRP1 attached to an NH2-terminal 6-His tag. IRP1 was expressed in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron metabolism; Iron-regulatory protein 1; Chaperonins; GroES; GroEL; Protein purification. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400003 Iron and glutathione at the crossroad of redox metabolism in neurons Biol. Res. ARACENA,PAULA; AGUIRRE,PABLA; MUÑOZ,PABLO; NÚÑEZ,MARCO T. Neurons, as non-dividing cells, encounter a myriad of stressful conditions throughout their lifespan. In particular, there is increasing evidence that iron progressively accumulates in the brain with age and that iron-induced oxidative stress is the cause of several forms of neurodegeneration. Here, we review recent evidence that gives support to the following notions: 1) neuronal iron accumulation leads to oxidative stress and cell death; 2) neuronal survival to iron accumulation associates with decreased expression of the iron import transporter DMT1 and increased expression of the efflux transporter IREG1; and 3) the adaptive process of neurons towards iron-induced oxidative stress includes a marked increase in both the expression of the catalytic... Tipo: Journal article Palavras-chave: Neurons; Iron metabolism; Iron accumulation; Oxidative stress; Glutathione metabolism; Neurodegeneration. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100017 Iron metabolism in domestic animais: a review MV&Z de Alencar, Nayro Xavier; Kohayagawa, Aguemi; Higino de Campos, Karla Conceição. Disorders of iron metabolism are both the main cause of iron deficiency anemia in small animals and the most commonly encountered deficiency in veterinary medicine. Iron metabolism assumes a fundamental role in the study of anemia. Although there are still many gaps, recent advances have resulted in a better understanding of the effects of iron imbalance. The objective of this paper was to present a review on iron metabolism in domestic animals, emphasizing the tests to evaluate this metabolism. Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron metabolism; Anemia; Hematology; Bone marrow Metabolismo del hierro; Anemia; Hematología; Médula ósea metabolismo do ferro; Anemia; Hematologia; Medula óssea. Ano: 2002 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3274 Sox2 function as a negative regulator to control HAMP expression Biol. Res. Song,Bin; Bian,Qi; Shao,Cheng-Hao; Liu,An-An; Jing,Wei; Liu,Rui; Zhang,Yi-Jie; Zhou,Ying-Qi; Li,Gang; Jin,Gang. BACKGROUND: Hepcidin, encoding by HAMP gene, is the pivotal regulator of iron metabolism, controlling the systemic absorption and transportation of irons from intracellular stores. Abnormal levels of HAMP expression alter plasma iron parameters and lead to iron metabolism disorders. Therefore,itis animportant goal to understand the mechanisms controlling HAMP gene expression. RESULTS: Overexpression of Sox2 decrease basal expression of HAMP or induced by IL-6 or BMP-2, whereas, knockdown of Sox2 can increase HAMP expression, furthermore, two potential Sox2-binding sites were identified within the human HAMP promoter. Indeed, luciferase experiments demonstrated that deletion of any Sox2-binding site impaired the negative regulation of Sox2 on HAMP promoter... Tipo: Journal article Palavras-chave: Sox2; Gene expression; Hepatocyte; HAMP; Iron metabolism. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100023 The prognostic value of the serum ferritin in a southern Brazilian cohort of patients with Gaucher disease Genet. Mol. Biol. Koppe,Tiago; Doneda,Divair; Siebert,Marina; Paskulin,Livia; Camargo,Matheus; Tirelli,Kristiane Michelin; Vairo,Filippo; Daudt,Liane; Schwartz,Ida Vanessa D.. Abstract The clinical utility of serum ferritin as a biomarker of disease severity and prognosis in Gaucher disease (GD) is still debated. Here, we aimed to evaluate ferritin and its relation to clinicolaboratory parameters of GD patients seen at the Reference Center for Gaucher Disease of Rio Grande do Sul, Brazil, so as to gather evidence on the utility of ferritin as a biomarker of this condition. A retrospective chart review was performed collecting pre-and posttreatment data from GD patients. Eighteen patients with ferritin levels available before and after treatment were included in the study. Nine of these participants were males, and seventeen had type I GD. All patients were given either enzyme replacement (n = 16) or substrate reduction therapy... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ferritin; Biomarkers; Gaucher disease; Iron metabolism. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100030 Registros recuperados: 7 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco